High-efficiency antibody discovery achieved with multiplexed microscopy

  title={High-efficiency antibody discovery achieved with multiplexed microscopy},
  author={Shelley Mettler Izquierdo and Stephanie Varela and Minha Park and Ellen J. Collarini and Daniel Lu and Shreya Pramanick and Joseph B. Rucker and L Lopalco and Rob Etches and William D. Harriman},
The analysis of secreted antibody from large and diverse populations of B cells in parallel at the clonal level can reveal desirable antibodies for diagnostic or therapeutic applications. By immobilizing B cells in microdroplets with particulate reporters, decoding and isolating them in a microscopy environment, we have recovered panels of antibodies with rare attributes to therapeutically relevant targets. The ability to screen up to 100 million cells in a single experiment can be fully… CONTINUE READING

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