High-efficiency and heritable gene targeting in mouse by transcription activator-like effector nucleases

  title={High-efficiency and heritable gene targeting in mouse by transcription activator-like effector nucleases},
  author={Zhongwei Qiu and Meizhen Liu and Zhaohua Chen and Yanjiao Shao and H. Pan and G. Wei and C. Yu and L. Zhang and Xia Li and P. Wang and H. Fan and B. Du and B. Liu and M. Liu and D. Li},
  journal={Nucleic Acids Research},
  pages={e120 - e120}
  • Zhongwei Qiu, Meizhen Liu, +12 authors D. Li
  • Published 2013
  • Medicine, Biology
  • Nucleic Acids Research
  • Transcription activator-like effector nucleases (TALENs) are a powerful new approach for targeted gene disruption in various animal models, but little is known about their activities in Mus musculus, the widely used mammalian model organism. Here, we report that direct injection of in vitro transcribed messenger RNA of TALEN pairs into mouse zygotes induced somatic mutations, which were stably passed to the next generation through germ-line transmission. With one TALEN pair constructed for each… CONTINUE READING
    GRK5-Knockout Mice Generated by TALEN-Mediated Gene Targeting
    • 3
    Highly Efficient Targeted Mutagenesis in Mice Using TALENs
    • 60
    • Open Access
    TALEN-mediated genome engineering to generate targeted mice
    • 22
    • Highly Influenced
    Endonucleases: new tools to edit the mouse genome.
    • 51
    • Open Access


    Publications referenced by this paper.
    Highly efficient generation of heritable zebrafish gene mutations using homo- and heterodimeric TALENs
    • 226
    • Open Access
    Efficient TALEN-mediated gene knockout in livestock
    • 496
    • Open Access
    Targeted Genome Modification in Mice Using Zinc-Finger Nucleases
    • 246
    • Highly Influential
    • Open Access
    Improved Somatic Mutagenesis in Zebrafish Using Transcription Activator-Like Effector Nucleases (TALENs)
    • 154
    • Open Access
    In vivo Genome Editing Using High Efficiency TALENs
    • 526
    • Open Access
    Heritable gene targeting in zebrafish using customized TALENs
    • 610
    Knockout Rats via Embryo Microinjection of Zinc-Finger Nucleases
    • 835
    • Open Access