High-efficiency and heritable gene targeting in mouse by transcription activator-like effector nucleases

@inproceedings{Qiu2013HighefficiencyAH,
  title={High-efficiency and heritable gene targeting in mouse by transcription activator-like effector nucleases},
  author={Zhongwei Qiu and Meizhen Liu and Zhaohua Chen and Yanjiao Shao and Hongjie Pan and Gaigai Wei and Chao Yu and Long Zhang and Xia Li and Ping Wang and Heng-Yu Fan and Bing Du and Bin Liu and Mingyao Liu and Dali Li},
  booktitle={Nucleic acids research},
  year={2013}
}
Transcription activator-like effector nucleases (TALENs) are a powerful new approach for targeted gene disruption in various animal models, but little is known about their activities in Mus musculus, the widely used mammalian model organism. Here, we report that direct injection of in vitro transcribed messenger RNA of TALEN pairs into mouse zygotes induced somatic mutations, which were stably passed to the next generation through germ-line transmission. With one TALEN pair constructed for each… CONTINUE READING
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