High efficiency DNA-mediated transformation of primate cells.

@article{Gorman1983HighED,
  title={High efficiency DNA-mediated transformation of primate cells.},
  author={Cornelia M. Gorman and Raji Padmanabhan and Bruce H Howard},
  journal={Science},
  year={1983},
  volume={221 4610},
  pages={551-3}
}
Tissue culture cells from several mammalian species, including three primate lines, were transfected with recombinant vectors carrying Escherichia coli xanthine-guanine phosphoribosyltransferase or Tn5 aminoglycoside phosphotransferase dominant selectable markers. Human HeLa and SV40-transformed xeroderma pigmentosum cells exhibited stable transformation frequencies of at least 10(-3) (0.1 percent). CV-1, an African green monkey kidney cell line, could be stably transformed with the… CONTINUE READING