High-efficiency DNA injection into a single human mesenchymal stem cell using a nanoneedle and atomic force microscopy.

@article{Han2008HighefficiencyDI,
  title={High-efficiency DNA injection into a single human mesenchymal stem cell using a nanoneedle and atomic force microscopy.},
  author={Sung-Woong Han and Chikashi Nakamura and Noriko Kotobuki and Ikuo Obataya and Hajime Ohgushi and Teruyuki Nagamune and Jun Miyake},
  journal={Nanomedicine : nanotechnology, biology, and medicine},
  year={2008},
  volume={4 3},
  pages={215-25}
}
We describe a low-invasive gene delivery method that uses an etched atomic force microscopy (AFM) tip or nanoneedle that can be inserted into a cell nucleus without causing cellular damage. The nanoneedle is 200 nm in diameter and 6 mum in length and is operated using an AFM system. The probabilities of insertion of the nanoneedle into human mesenchymal stem cells (MSCs) and human embryonic kidney cells (HEK293) were higher than those of typical microinjection capillaries. A plasmid containing… CONTINUE READING
Highly Cited
This paper has 66 citations. REVIEW CITATIONS
31 Extracted Citations
29 Extracted References
Similar Papers

Citing Papers

Publications influenced by this paper.
Showing 1-10 of 31 extracted citations

66 Citations

01020'10'12'14'16'18
Citations per Year
Semantic Scholar estimates that this publication has 66 citations based on the available data.

See our FAQ for additional information.

Referenced Papers

Publications referenced by this paper.
Showing 1-10 of 29 references

Similar Papers

Loading similar papers…