High-density transient gene expression in suspension-adapted 293 EBNA1 cells.

  title={High-density transient gene expression in suspension-adapted 293 EBNA1 cells.},
  author={X. Sun and Hui Ching Hia and Peen Ern Goh and Miranda Gek Sim Yap},
  journal={Biotechnology and bioengineering},
  volume={99 1},
Large-scale transient gene expression (TGE) in mammalian cells is an attractive method to rapidly produce recombinant proteins for pre-clinical studies, with some processes reported to reach 100 L. However, the yield remains low, hardly over 20 mg protein/L, mainly because the current TGEs have been performed at low cell density (approximately 5 x 10(5) cells/mL). In this study, the strategy to improve TGE focuses on facilitating transfection at high cell density. A high-density perfusion… CONTINUE READING

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