High density lipoprotein cholesterol as a mechanistic probe for the side chain cleavage reaction.

Abstract

Cholesterol side chain cleavage reaction catalyzed by purified cytochrome P-450scc was stimulated 4-5 fold when cholesterol in rat high density lipoprotein was used as a substrate as compared to the case where cholesterol plus 0.1% Emulgen 911 was used. In the case of the cholesterol-Emulgen system, the Vmax value of activity was not obtained even when a 20… (More)

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