High density culture of Coptis japonica cells increases berberine production

  title={High density culture of Coptis japonica cells increases berberine production},
  author={Kōichi Matsubara and Shigekazu Kitani and Toshihiro Yoshioka and Teijiro Morimoto and Yasuhiro Fujita and Yasuyuki Yamada},
  journal={Journal of Chemical Technology \& Biotechnology},
A high density culture method was devised to improve the yield of berberine from highly productive cells of Coptis japonica. By adjusting aeration and stirring, Coptis cells were cultured at densities of up to 75 g dm−3 (dry weight) in a culture tank fitted with a hollow-paddle type stirrer. Whereas a maximum density of 30 g dm−3 of C. japonica cells could be used in ordinary batch culture, 48 g dm−3 could be used in a fed-batch culture in which the amounts of the nutrients in the medium… Expand
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