High-contrast imaging of fluorescent protein FRET by fluorescence polarization microscopy.

@article{Rizzo2005HighcontrastIO,
  title={High-contrast imaging of fluorescent protein FRET by fluorescence polarization microscopy.},
  author={Mark A Rizzo and David W. Piston},
  journal={Biophysical journal},
  year={2005},
  volume={88 2},
  pages={L14-6}
}
Detection of Forster resonance energy transfer (FRET) between fluorescent protein labeled targets is a valuable strategy for measurement of protein-protein interactions and other intracellular processes. Despite the utility of FRET, widespread application of this technique to biological problems and high-throughput screening has been limited by low-contrast measurement strategies that rely on the detection of sensitized emission or photodestruction of the sample. Here we report a FRET detection… CONTINUE READING

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