High-content super-resolution imaging of live cell by uPAINT.


In this chapter, we present the uPAINT method (Universal Point Accumulation Imaging in Nanoscale Topography), a simple single-molecule super-resolution method which can be implemented on any wide field fluorescence microscope operating in oblique illumination. The key feature of uPAINT lies in recording high numbers of single molecules at the surface of a… (More)
DOI: 10.1007/978-1-62703-137-0_7

Cite this paper

@article{Giannone2013HighcontentSI, title={High-content super-resolution imaging of live cell by uPAINT.}, author={Gr{\'e}gory Giannone and Eric Hosy and Jean-Baptiste Sibarita and Daniel Choquet and Laurent Cognet}, journal={Methods in molecular biology}, year={2013}, volume={950}, pages={95-110} }