High content screening of CXCR2-dependent signalling pathways.

  title={High content screening of CXCR2-dependent signalling pathways.},
  author={Michael Wolff and Simone Kredel and Dorothea Haasen and Joerg Wiedenmann and Gerd Ulrich Nienhaus and Barbara Kistler and Franz Oswald and Ralf Heilker},
  journal={Combinatorial chemistry & high throughput screening},
  volume={13 1},
Stimulation of CXC-type chemokine receptor 2 (CXCR2)-transfected cells by Gro-alpha or IL-8 induced (i) CXCR2 internalization, (ii) phosphorylation of ERK1/2 (pERK) and (iii) translocation of nuclear factor of activated T cells (NFAT) into the nucleus. Employing high content screening (HCS; i.e. fluorimetric imaging combined with image analysis) these three ligand-induced events were quantified by using a CXCR2-specific antibody, an antibody recognizing phosphorylated ERK1/2 (pERK) and a red… CONTINUE READING

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