High-content screening and profiling of drug activity in an automated centrosome-duplication assay.

@article{Perlman2005HighcontentSA,
  title={High-content screening and profiling of drug activity in an automated centrosome-duplication assay.},
  author={Zachary E. Perlman and Timothy J Mitchison and Thomas U. Mayer},
  journal={Chembiochem : a European journal of chemical biology},
  year={2005},
  volume={6 1},
  pages={145-51}
}
Maintenance of centrosome number is essential for cell-cycle progression and genomic stability, but investigation of this regulation has been limited by assay difficulty. We present a fully automated image-based centrosome-duplication assay that is accurate and robust enough for both careful cell-biology studies and high-throughput screening, and employ this assay in a series of chemical-genetic studies. We observe that a simple cytometric profiling strategy, which is based on organelle size… CONTINUE READING
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