High-content profiling of cell responsiveness to graded substrates based on combinyatorially variant polymers.

@article{Liu2009HighcontentPO,
  title={High-content profiling of cell responsiveness to graded substrates based on combinyatorially variant polymers.},
  author={Er Liu and Matthew D Treiser and Hiral R Patel and Hak-Joon Sung and Kristen E. Roskov and Joachim Kohn and Matthew L Becker and Prabhas V. Moghe},
  journal={Combinatorial chemistry & high throughput screening},
  year={2009},
  volume={12 7},
  pages={
          646-55
        }
}
We have developed a novel approach combining high information and high throughput analysis to characterize cell adhesive responses to biomaterial substrates possessing gradients in surface topography. These gradients were fabricated by subjecting thin film blends of tyrosine-derived polycarbonates, i.e. poly(DTE carbonate) and poly(DTO carbonate) to a gradient temperature annealing protocol. Saos-2 cells engineered with a green fluorescent protein (GFP) reporter for farnesylation (GFP-f) were… CONTINUE READING