High-content imaging and analysis of pluripotent stem cell-derived cardiomyocytes.

Abstract

Human pluripotent stem cells (hPSC) are investigated as a source of authentic human cardiac cells for drug discovery and toxicological tests. Cell-based assays using automated fluorescence imaging platform and high-content analysis characterize hypertrophic and toxicity profiles of compounds in hPSC-derived cardiomyocytes (hPSC-CM) at the cellular and subcellular levels. In purified population of hPSC-CM loaded with cell tracer probe and cell death markers, both hypertrophic and toxicity profiles can be assessed in live cardiomyocyte cultures. Alternatively, in non-purified cultures of hPSC-CM, hypertrophy, proliferation, and cell death assays can be performed specifically in the cardiomyocyte subpopulation using antibodies directed against cardiac proteins and a combination of cell death- and proliferation-specific fluorescent probes.

DOI: 10.1007/7651_2013_25

Cite this paper

@article{Fldes2013HighcontentIA, title={High-content imaging and analysis of pluripotent stem cell-derived cardiomyocytes.}, author={G{\'a}bor F{\"{o}ldes and Maxime Mioulane}, journal={Methods in molecular biology}, year={2013}, volume={1052}, pages={29-39} }