High-content functional screen to identify proteins that correct F508del-CFTR function.

@article{TrzciskaDaneluti2009HighcontentFS,
  title={High-content functional screen to identify proteins that correct F508del-CFTR function.},
  author={Agata M Trzcińska-Daneluti and Diane Ly and Lise Huynh and Chong Jiang and Christopher Fladd and Daniela Rotin},
  journal={Molecular & cellular proteomics : MCP},
  year={2009},
  volume={8 4},
  pages={780-90}
}
Cystic Fibrosis is caused by mutations in CFTR, with a deletion of a phenylalanine at position 508 (F508del-CFTR) representing the most common mutation. The F508del-CFTR protein exhibits a trafficking defect and is retained in the endoplasmic reticulum. Here we describe the development of a high-content screen based on a functional assay to identify proteins that correct the F508del-CFTR defect. Using a HEK293 MSR GripTite cell line that stably expresses F508del-CFTR, we individually co… CONTINUE READING
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