High-content classification of nucleocytoplasmic import or export inhibitors.

  title={High-content classification of nucleocytoplasmic import or export inhibitors.},
  author={Yong-Jun Kwon and Auguste Genovesio and Nam Youl Kim and Hi Chul Kim and Sungyong Jung and Brigitte David-Watine and Ulf Nehrbass and Neil Emans},
  journal={Journal of biomolecular screening},
  volume={12 5},
Transcription factors of the nuclear factor kappa B family are the paradigm for signaling dependent nuclear translocation and are ideally suited to analysis through image-based chemical genetic screening. The authors describe combining high-content image analysis with a compound screen to identify compounds affecting either nuclear import or export. Validation in silico and in vitro determined an EC(50) for the nuclear export blocker leptomycin B of 2.4 ng/mL (4.4 nM). The method demonstrated… CONTINUE READING