High cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties

@inproceedings{MenndezBravo2016HighCD,
  title={High cell density production of multimethyl-branched long-chain esters in Escherichia coli and determination of their physicochemical properties},
  author={Sim{\'o}n M Men{\'e}ndez-Bravo and Julia Roulet and Martin Sabatini and Santiago Comba and Robert O. Dunn and Hugo Gramajo and Ana Arabolaza},
  booktitle={Biotechnology for biofuels},
  year={2016}
}
BACKGROUND Microbial synthesis of oleochemicals derived from native fatty acid (FA) metabolism has presented significant advances in recent years. Even so, native FA biosynthetic pathways often provide a narrow variety of usually linear hydrocarbons, thus yielding end products with limited structural diversity. To overcome this limitation, we took advantage of a polyketide synthase-based system from Mycobacterium tuberculosis and developed an Escherichia coli platform with the capacity to… CONTINUE READING
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