High cell density fermentation of recombinant Escherichia coli expressing human interferon alpha 1

@article{Riesenberg1990HighCD,
  title={High cell density fermentation of recombinant Escherichia coli expressing human interferon alpha 1},
  author={Dieter Riesenberg and Klaus Menzel and Volkmar Schulz and Klaus Schumann and Gerhard Veith and Georg Zuber and Wolfgang A. Knorre},
  journal={Applied Microbiology and Biotechnology},
  year={1990},
  volume={34},
  pages={77-82}
}
A defined medium was developed which, by means of a specific fed-batch mode, allows growth of the recombinant Escherichia coli strain TG1 (pBB210) up to a cell density of 60 g dry weight/l. Apart from glucose and aqueous ammonia fed as carbon and nitrogen sources, it was unnecessary to supply other nutrients or O2-enriched air. Aqueous ammonia also served for pH control. The pO2 level was kept at 20% saturation via closed-loop controls operating the two output variables of stirrer speed and… CONTINUE READING

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