High catalytic activity of human cytochrome P450 co-expressed with human NADPH-cytochrome P450 reductase in Escherichia coli.

@article{Iwata1998HighCA,
  title={High catalytic activity of human cytochrome P450 co-expressed with human NADPH-cytochrome P450 reductase in Escherichia coli.},
  author={H. Iwata and Ken-Ichi Fujita and H. Kushida and A. Suzuki and Y. Konno and K. Nakamura and A. Fujino and T. Kamataki},
  journal={Biochemical pharmacology},
  year={1998},
  volume={55 8},
  pages={
          1315-25
        }
}
Forms of human cytochrome P450 (P450 or CYP), such as CYP1A1, CYP1A2, CYP2A6, CYP2C8, CYP2C9, CYP2C19, CYP2D6, CYP2E1, and CYP3A4, were expressed or co-expressed together with human NADPH-P450 reductase in Escherichia coli. When P450 was expressed alone in E. coli, the expression level of holo-P450 ranged from 310 to 1620 nmol/L of culture. The expression level of holo-P450 decreased by co-expression with the reductase, and the level ranged from 66 to 381 nmol/L of culture. The expression level… Expand
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