High albumin production by multicellular spheroids of adult rat hepatocytes formed in the pores of polyurethane foam

@article{Matsushita1991HighAP,
  title={High albumin production by multicellular spheroids of adult rat hepatocytes formed in the pores of polyurethane foam},
  author={Taku Matsushita and Hiroyuki Ijima and Norio Koide and Kazumori Funatsu},
  journal={Applied Microbiology and Biotechnology},
  year={1991},
  volume={36},
  pages={324-326}
}
Adult rat hepatocytes formed spherical multicellular aggregates (spheroids) when they were cultured in the pores of polyurethane foam (PUF). The diameter of the spheroids was within the range 100–200 μm. These spheroids partly attached and immobilized in the PUF pores for at least 2 weeks. The albumin production rate by the spheroids increased up to 17.0 μg/106 nuclei per day during the first 6 days and maintained at a high level for 2 weeks. In contrast, the albumin production rate by the… CONTINUE READING

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References

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Showing 1-10 of 11 references

Enzyme production by high density culture of mammalian cells using polyurethane foam

  • T Matsushita, K Funatsu
  • 1990

Enzyme production by high density culture of mammalian cells using polyurethane foam as a culture substrate

  • T Matsushita, K Funatsu
  • 1990

Bilirubin conjugation by multicellular spheroid of adult rat hepatocytes in primary culture

  • M Kawaguchi, N Koide, K Sakaguchi, K Asano, M Moil, T Tsuji
  • Jpn J Artif Organs
  • 1989
1 Excerpt

The biosynthesis of rat serum albumin

  • Peter T, JC Peter
  • J'Biol Chem
  • 1972

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