High-affinity binding sites involved in the import of porin into mitochondria.

@article{Pfaller1987HighaffinityBS,
  title={High-affinity binding sites involved in the import of porin into mitochondria.},
  author={R. Pfaller and Walter Neupert},
  journal={The EMBO journal},
  year={1987},
  volume={6 9},
  pages={2635-42}
}
The specific recognition by mitochondria of the precursor of porin and the insertion into the outer membrane were studied with a radiolabeled water-soluble form of porin derived from the mature protein. High-affinity binding sites had a number of 5-10 pmol/mg mitochondrial protein and a ka of 1-5 X 10(8) M-1. Binding was abolished after trypsin pretreatment of mitochondria indicating that binding sites were of protein-aceous nature. Specifically bound porin could be extracted at alkaline pH but… CONTINUE READING