High-affinity binding sites for heparin generated on leukocytes during apoptosis arise from nuclear structures segregated during cell death.

@article{Gebska2002HighaffinityBS,
  title={High-affinity binding sites for heparin generated on leukocytes during apoptosis arise from nuclear structures segregated during cell death.},
  author={Milena A Gebska and Ian Titley and Hugh F. Paterson and Ricardo M Morilla and Derek C Davies and ALICJA M. GRUSZKA-WESTWOOD and Vijay Vir Kakkar and Suzanne A. Eccles and Michael F. Scully},
  journal={Blood},
  year={2002},
  volume={99 6},
  pages={2221-7}
}
During cell death of human cultured leukocytes (Jurkat, HL-60, THP-1, U937) and freshly prepared leukocytes, we observed a greater than 100-fold increase in the affinity of apoptotic and necrotic cells for fluorescein isothiocyanate (FITC)-heparin in comparison with live cells. Binding of FITC-heparin was reversed in the presence of high ionic strength, unlabeled heparan sulfate, and heparin and pentosan polysulfate, but not in the presence of chondroitin and dermatan sulfates. During the… CONTINUE READING

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