High-affinity binders selected from designed ankyrin repeat protein libraries

@article{Binz2004HighaffinityBS,
  title={High-affinity binders selected from designed ankyrin repeat protein libraries},
  author={H. Kaspar Binz and Patrick Amstutz and Andreas Kohl and Michael T. Stumpp and Christophe Briand and Patrik Forrer and Markus G. Grütter and Andreas Pl{\"u}ckthun},
  journal={Nature Biotechnology},
  year={2004},
  volume={22},
  pages={575-582}
}
We report here the evolution of ankyrin repeat (AR) proteins in vitro for specific, high-affinity target binding. Using a consensus design strategy, we generated combinatorial libraries of AR proteins of varying repeat numbers with diversified binding surfaces. Libraries of two and three repeats, flanked by 'capping repeats,' were used in ribosome-display selections against maltose binding protein (MBP) and two eukaryotic kinases. We rapidly enriched target-specific binders with affinities in… 
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