High affinity ScFvs from a single rabbit immunized with multiple haptens.

@article{Li2000HighAS,
  title={High affinity ScFvs from a single rabbit immunized with multiple haptens.},
  author={Yong Li and William Cockburn and J. B. Kilpatrick and Garry C. Whitelam},
  journal={Biochemical and biophysical research communications},
  year={2000},
  volume={268 2},
  pages={
          398-404
        }
}
We report the generation of single-chain Fv (scFv) fragments with high affinities against four different hapten molecules from a single immunised rabbit. The rabbit was immunised with a mixture of protein conjugates of four different haptens, namely the herbicide mecoprop and derivatives of the herbicides atrazine, simazine, and isoproturon. An scFv phage display library was constructed, and several scFvs with high affinity against each hapten were isolated. For each hapten, a single binder was… CONTINUE READING
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