High-affinity RNA ligands to human alpha-thrombin

  title={High-affinity RNA ligands to human alpha-thrombin},
  author={Mark F. Kubik and Andrew W. Stephens and Dan Schneider and Richard A. Marlar and Diane Tasset},
  journal={Nucleic acids research},
  volume={22 13},
Systematic Evolution of Ligands by EXponential enrichment (SELEX) was used to isolate from a population of 10(13) RNA molecules two classes of high affinity RNAs that bind specifically to human alpha-thrombin. Class I RNAs are represented by a 24-nucleotide RNA (RNA 16.24), and class II RNAs are represented by a 33-nucleotide RNA (RNA 27.33). RNA 16.24 inhibits thrombin-catalyzed fibrin clot formation in vitro. Secondary structures are proposed for these RNAs, revealing a novel stem-loop… CONTINUE READING


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