High activity expression of D-amino acid oxidase in Escherichia coli by the protein expression rate optimization.

@article{Hou2013HighAE,
  title={High activity expression of D-amino acid oxidase in Escherichia coli by the protein expression rate optimization.},
  author={Jixian Hou and Yangqiu Liu and Qiang Li and Jichu Yang},
  journal={Protein expression and purification},
  year={2013},
  volume={88 1},
  pages={120-6}
}
Two categories of expression systems with different promoters and substitutive ribosome binding site region (RBS) were constructed in order to improve the soluble expression of d-amino acid oxidase (DAAO) basing on the hypothesis that the optimal promoter and suitable RBS would provide the recombinant expression system with better matched expression rate, which served as key factor to the heterogenous synthesis of soluble protein. The results showed that with rational promoter recombination and… CONTINUE READING

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