High-Throughput Spectral and Lifetime-Based FRET Screening in Living Cells to Identify Small-Molecule Effectors of SERCA.

@article{Schaaf2017HighThroughputSA,
  title={High-Throughput Spectral and Lifetime-Based FRET Screening in Living Cells to Identify Small-Molecule Effectors of SERCA.},
  author={Tory M Schaaf and Kurt C Peterson and Benjamin D Grant and Prachi Bawaskar and Samantha L. Yuen and Ji Li and Joseph M. Muretta and Gregory D. Gillispie and David D Thomas},
  journal={SLAS discovery : advancing life sciences R & D},
  year={2017},
  volume={22 3},
  pages={262-273}
}
A robust high-throughput screening (HTS) strategy has been developed to discover small-molecule effectors targeting the sarco/endoplasmic reticulum calcium ATPase (SERCA), based on a fluorescence microplate reader that records both the nanosecond decay waveform (lifetime mode) and the complete emission spectrum (spectral mode), with high precision and speed… CONTINUE READING