High Throughput Screening of Esterases, Lipases and Phospholipases in Mutant and Metagenomic Libraries: A Review.

@article{PeaGarca2016HighTS,
  title={High Throughput Screening of Esterases, Lipases and Phospholipases in Mutant and Metagenomic Libraries: A Review.},
  author={Carlina Pe{\~n}a-Garc{\'i}a and M. Llanos Martinez-Martinez and Dolores Reyes-Duarte and Manuel Ferrer},
  journal={Combinatorial chemistry & high throughput screening},
  year={2016},
  volume={19 8},
  pages={
          605-615
        }
}
Nowadays, enzymes can be efficiently identified and screened from metagenomic resources or mutant libraries. A set of a few hundred new enzymes can be found using a simple substrate within few months. Hence, the establishment of collections of enzymes is no longer a big hurdle. However, a key problem is the relatively low rate of positive hits and that a… CONTINUE READING