High-Throughput Mapping of a Dynamic Signaling Network in Mammalian Cells

  title={High-Throughput Mapping of a Dynamic Signaling Network in Mammalian Cells},
  author={Miriam Barrios-Rodiles and Kevin R. Brown and Barish Ozdamar and Rohit Bose and Zhongda Chris Liu and Robert S. Donovan and Fukiko Shinjo and Yongmei Liu and Joanna Dembowy and Ian W. F. Taylor and Valbona Luga and Natasa Przulj and Mark Robinson and Harukazu Suzuki and Yoshihide Hayashizaki and Igor Jurisica and Jeffrey L. Wrana},
  pages={1621 - 1625}
Signaling pathways transmit information through protein interaction networks that are dynamically regulated by complex extracellular cues. We developed LUMIER (for luminescence-based mammalian interactome mapping), an automated high-throughput technology, to map protein-protein interaction networks systematically in mammalian cells and applied it to the transforming growth factor–β (TGFβ) pathway. Analysis using self-organizing maps and k-means clustering identified links of the TGFβ pathway to… 

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