High Throughput Automated Allele Frequency Estimation by Pyrosequencing


Pyrosequencing is a DNA sequencing method based on the principle of sequencing-by-synthesis and pyrophosphate detection through a series of enzymatic reactions. This bioluminometric, real-time DNA sequencing technique offers unique applications that are cost-effective and user-friendly. In this study, we have combined a number of methods to develop an… (More)
DOI: 10.1371/journal.pone.0002693


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@article{Doostzadeh2008HighTA, title={High Throughput Automated Allele Frequency Estimation by Pyrosequencing}, author={Julie Doostzadeh and Shadi Shokralla and Farnaz Absalan and Roxana Jalili and Sharareh Mohandessi and James Langston and Ronald W. Davis and Mostafa Ronaghi and Baback Gharizadeh}, journal={PLoS ONE}, year={2008}, volume={3}, pages={363 - 401} }