High-Throughput, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing

Abstract

A scalable and high-throughput method to identify precise subcellular localization of endogenous proteins is essential for integrative understanding of a cell at the molecular level. Here, we developed a simple and generalizable technique to image endogenous proteins with high specificity, resolution, and contrast in single cells in mammalian brain tissue… (More)
DOI: 10.1016/j.cell.2016.04.044

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Cite this paper

@article{Mikuni2016HighThroughputHM, title={High-Throughput, High-Resolution Mapping of Protein Localization in Mammalian Brain by In Vivo Genome Editing}, author={Takayasu Mikuni and Jun Nishiyama and Ye Sun and Naomi Kamasawa and Ryohei Yasuda}, journal={Cell}, year={2016}, volume={165}, pages={1803-1817} }