High Stability of Recombinant Proteins Expressed in Tobacco Chloro- plasts


The green fluorescent protein and the non-toxic subunit of cholera toxin were expressed in tobacco chloroplasts. Both recombinant proteins accumulated to levels greater than 20% of the total soluble protein. We did not observe light-dependent induction of gene expression despite employing the promoter and 5 ́-UTR from the psbA gene. Both proteins were… (More)