High Sensitivity of Zn2+ Insulin to Metal-Catalyzed Oxidation: Detection of 2-Oxo-Histidine by Tandem Mass Spectrometry

@article{Hovorka2002HighSO,
  title={High Sensitivity of Zn2+ Insulin to Metal-Catalyzed Oxidation: Detection of 2-Oxo-Histidine by Tandem Mass Spectrometry},
  author={Susan W. Hovorka and Homigol Biesiada and Todd D. Williams and Andreas F. R. H{\"u}hmer and Christian Sch{\"o}neich},
  journal={Pharmaceutical Research},
  year={2002},
  volume={19},
  pages={530-537}
}
Purpose. To establish the sensitivity of Zn2+ insulin (Zn2+-INS) to metal-catalyzed oxidation (MCO) and to use tandem mass spectrometry/mass spectroscopy (MS/MS) for the identification and quantification of 2-oxo-histidine at 5HisB and 10HisB upon the MCO of INS. Methods. Zn2+-INS was exposed to Cu2+/ascorbate-induced MCO. Products were analyzed by reverse-phase high-performance liquid chromatography, electrospray ionization mass spectrometry (ESI-MS) and MS/MS, sodium dodecylsulfate… CONTINUE READING

References

Publications referenced by this paper.
Showing 1-10 of 30 references

basic sciences to therapeutic uses

Y. W. Chien. Human insulin
Drug Dev. Indus. Pharm. 22:753–789 • 1996
View 5 Excerpts
Highly Influenced

Site-specific oxidation of histidine residues in glycated insulin mediated by Cu2+.

European journal of biochemistry • 1994
View 5 Excerpts
Highly Influenced

Interpreting Protein Mass Spectra

A. P. Snyder
2000
View 1 Excerpt

Insulin—structure, function, design

D. Brandenburg
Exp. Clin. Endocrinol. Diabetes • 1999

Principles of Physical Biochemistry, Prentice-Hall, Inc

K. Van Holde, W. C. Johnson, P. S. Ho
Upper Saddle River, NJ, • 1998

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