High Sensitivity In-Cell EPR Distance Measurements on Proteins using an Optimized Gd(III) Spin Label.

@article{Yang2018HighSI,
  title={High Sensitivity In-Cell EPR Distance Measurements on Proteins using an Optimized Gd(III) Spin Label.},
  author={Y. Yang and Feng Yang and Yan-Jun Gong and Thorsten Bahrenberg and Akiva Feintuch and Xuncheng Su and Daniella Goldfarb},
  journal={The journal of physical chemistry letters},
  year={2018},
  volume={9 20},
  pages={
          6119-6123
        }
}
Distance measurements by electron-electron double resonance (DEER) carried out on spin-labeled proteins delivered into cells provide new insights into the conformational states of proteins in their native environment. Such measurements depend on spin labels that exhibit high redox stability and high DEER sensitivity. Here we present a new Gd(III)-based spin label, BrPSPy-DO3A-Gd(III), which was derived from an earlier label, BrPSPy-DO3MA-Gd(III), by removing the methyl group from the methyl… 
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