High Resolution Melting Analysis Is a More Sensitive and Effective Alternative to Gel-Based Platforms in Analysis of SSR – An Example in Citrus

@inproceedings{Distefano2012HighRM,
  title={High Resolution Melting Analysis Is a More Sensitive and Effective Alternative to Gel-Based Platforms in Analysis of SSR – An Example in Citrus},
  author={Gaetano Distefano and Marco Caruso and Stefano La Malfa and Alessandra Gentile and Shu-Biao Wu},
  booktitle={PloS one},
  year={2012}
}
High resolution melting curve analysis (HRM) has been used as an efficient, accurate and cost-effective tool to detect single nucleotide polymorphisms (SNPs) or insertions or deletions (INDELs). However, its efficiency, accuracy and applicability to discriminate microsatellite polymorphism have not been extensively assessed. The traditional protocols used for SSR genotyping include PCR amplification of the DNA fragment and the separation of the fragments on electrophoresis-based platform… CONTINUE READING
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Microsatellite high resolution melting (SSR-HRM) analysis for authenticity testing of protected designation of origin (PDO) sweet cherry products

I Ganopoulos, A Argiriou, A Tsaftaris
Food Control • 2011
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