High-Resolution Melt Curve Analysis in Cancer Mutation Screen.


High-resolution melt (HRM) curve analysis is a PCR-based assay that identifies sequence alterations based on subtle variations in the melting curves of mutated versus wild-type DNA sequences. HRM analysis is a high-throughput, sensitive, and efficient alternative to Sanger sequencing and is used to assess for mutations in clinically important genes involved… (More)
DOI: 10.1007/978-1-4939-3360-0_7


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