High-Resolution Imaging of Redox Signaling in Live Cells Through an Oxidation-Sensitive Yellow Fluorescent Protein

@article{Maulucci2008HighResolutionIO,
  title={High-Resolution Imaging of Redox Signaling in Live Cells Through an Oxidation-Sensitive Yellow Fluorescent Protein},
  author={G. Maulucci and V. Labate and M. Mele and Emiliano Panieri and G. Arc{\`o}vito and T. Galeotti and H. {\O}stergaard and J. R. Winther and M. De Spirito and G. Pani},
  journal={Science Signaling},
  year={2008},
  volume={1},
  pages={pl3 - pl3}
}
  • G. Maulucci, V. Labate, +7 authors G. Pani
  • Published 2008
  • Biology, Medicine
  • Science Signaling
  • Measure and visualize intracellular changes in redox state. We present the application of a redox-sensitive mutant of the yellow fluorescent protein (rxYFP) to image, with elevated sensitivity and high temporal and spatial resolution, oxidative responses of eukaryotic cells to pathophysiological stimuli. The method presented, based on the ratiometric quantitation of the distribution of fluorescence by confocal microscopy, allows us to draw real-time “redox maps” of adherent cells and to score… CONTINUE READING
    46 Citations

    Topics from this paper

    Fluorescent protein-based redox probes.
    • 398
    Dissecting Redox Biology Using Fluorescent Protein Sensors.
    • 150
    Fluorescent in vivo imaging of reactive oxygen species and redox potential in plants
    • 14
    • PDF
    Genetically encoded fluorescent redox sensors.
    • 102
    Genetically encoded reactive oxygen species (ROS) and redox indicators
    • 29

    References

    SHOWING 1-10 OF 24 REFERENCES
    Real-time imaging of the intracellular glutathione redox potential
    • 566
    • PDF
    Investigating Mitochondrial Redox Potential with Redox-sensitive Green Fluorescent Protein Indicators*
    • 793
    • PDF
    Measuring intracellular redox conditions using GFP-based sensors.
    • 61
    Creating new fluorescent probes for cell biology
    • 1,370
    • PDF
    Fluorescence-sensing methods.
    • 41
    Redox-dependent transcriptional regulation.
    • 381
    • PDF