High-Resolution Imaging of Redox Signaling in Live Cells Through an Oxidation-Sensitive Yellow Fluorescent Protein

@article{Maulucci2008HighResolutionIO,
  title={High-Resolution Imaging of Redox Signaling in Live Cells Through an Oxidation-Sensitive Yellow Fluorescent Protein},
  author={Giuseppe Maulucci and Valentina Labate and Marina Mele and Emiliano Panieri and Giuseppe Arc{\`o}vito and Tommaso Galeotti and Henrik {\O}stergaard and Jakob Rahr Winther and Marco De Spirito and Giovambattista Pani},
  journal={Science Signaling},
  year={2008},
  volume={1},
  pages={pl3 - pl3}
}
Measure and visualize intracellular changes in redox state. We present the application of a redox-sensitive mutant of the yellow fluorescent protein (rxYFP) to image, with elevated sensitivity and high temporal and spatial resolution, oxidative responses of eukaryotic cells to pathophysiological stimuli. The method presented, based on the ratiometric quantitation of the distribution of fluorescence by confocal microscopy, allows us to draw real-time “redox maps” of adherent cells and to score… Expand
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