High Level Secretion of a Humanized Bispecific Diabody from Escherichia coli

@article{Zhu1996HighLS,
  title={High Level Secretion of a Humanized Bispecific Diabody from Escherichia coli},
  author={Zhenping Zhu and Gerardo A. Zapata and Refaat Shalaby and Brad Snedecor and Han Chen and Paul H Carter},
  journal={Bio/Technology},
  year={1996},
  volume={14},
  pages={192-196}
}
Clinical development of bispecific antibodies (BsAb) has been effectively stymied by the lack of efficient production methods. We therefore attempted to produce a humanized BsAb fragment using an expression system that has proved very successful for secretion of monospecific Ab fragments from E. coli. An anti-p185HER2/anti-CD3 BsF(ab′)2 was first recast into the diabody format and then periplasmically secreted from E. coli grown to high cell density in a fermentor. The diabody was recovered in… CONTINUE READING