High-Level Expression of Bacillus naganoensis Pullulanase from Recombinant Escherichia coli with Auto-Induction: Effect of lac Operator

@inproceedings{Nie2013HighLevelEO,
  title={High-Level Expression of Bacillus naganoensis Pullulanase from Recombinant Escherichia coli with Auto-Induction: Effect of lac Operator},
  author={Yao Nie and Wei Yan and Yan Xu and Wen Bo Chen and Xiao Qing Mu and Xinye Wang and Rong Xiao},
  booktitle={PloS one},
  year={2013}
}
Pullulanase plays an important role in specific hydrolysis of branch points in amylopectin and is generally employed as an important enzyme in starch-processing industry. So far, however, the production level of pullulanase is still somewhat low from wide-type strains and even heterologous expression systems. Here the gene encoding Bacillus naganoensis pullulanase was amplified and cloned. For expression of the protein, two recombinant systems, Escherichia coli BL21(DE3)/pET-20b(+)-pul and E… CONTINUE READING
9 Citations
44 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-9 of 9 extracted citations

References

Publications referenced by this paper.
Showing 1-10 of 44 references

Improved high thermal stability of pullulanase from a newly isolated thermophilic Bacillus sp. AN-7

  • A Kunamneni, S Singh
  • Enzyme Microb Technol
  • 2006
Highly Influential
4 Excerpts

Thermostable pullulanase type I from new isolated Bacillus thermoleovorans US105: cloning, sequencing and expression of the gene in E. coli

  • E Ben Messaoud, Y Ben Ammar, L Mellouli, S Bejar
  • Enzyme Microb Technol
  • 2002
Highly Influential
4 Excerpts

Use of glucose to control basal expression in the pET system innovations

  • R Novy, B Morris
  • BioTechniques
  • 2001
Highly Influential
6 Excerpts

Statistical optimization of pullulanase production by Raoultella planticola DSMZ 4617 using sago starch as carbon and peptone as nitrogen sources

  • LS Hii, M Rosfarizan, TC Ling, AB Ariff
  • Food Bioprocess Technol
  • 2012
3 Excerpts

Continuous hydrolysis of pullulan using covalently immobilized pullulanase in a packed bed reactor

  • RS Singh, GK Saini, JF Kennedy
  • Carbohydr Polym
  • 2011
1 Excerpt

Similar Papers

Loading similar papers…