High Efficiency Transformation of Intact Yeast Cells by Electric Field Pulses

  title={High Efficiency Transformation of Intact Yeast Cells by Electric Field Pulses},
  author={Eliane Meilhoc and J. Masson and Justin Teissi{\'e}},
We have developed an efficient method that electrically introduces DNA into intact yeast cells. Saccharomyces cerevisiae was used as a model in order to optimize the transformation protocol. Transformation efficiencies of 107 transformants/μg of plasmid DNA were obtained with a square wave electric pulse of 2.7 kV/cm during 15 milliseconds. The technique is simple and rapid. Even small quantities of DNA (100 pg) can be used to transform 108 cells. Important parameters are the pulse field… CONTINUE READING

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