High Efficacy of Clonal Growth and Expansion of Adult Neural Stem Cells

@article{Wachs2003HighEO,
  title={High Efficacy of Clonal Growth and Expansion of Adult Neural Stem Cells},
  author={Frank-Peter Wachs and Sebastien Couillard-Despres and Maren Engelhardt and Daniel Wilhelm and Sonja Ploetz and Maurice Vroemen and Johanna Kaesbauer and Goekhan Uyanik and Jochen Klucken and Claudia Karl and Johanna Tebbing and Clive Svendsen and Norbert J Weidner and H. Georg Kuhn and J{\"u}rgen F. H. Winkler and Ludwig Aigner},
  journal={Laboratory Investigation},
  year={2003},
  volume={83},
  pages={949-962}
}
Neural stem cells (NSCs) from the adult central nervous system are currently being investigated for their potential use in autologous cell replacement strategies. High expansion rates of NSCs in culture are crucial for the generation of a sufficient amount of cells needed for transplantation. Here, we describe efficient growth of adult NSCs in Neurobasal medium containing B27 supplement under clonal and low-density conditions in the absence of serum or conditioned medium. Expansion of up to 15… CONTINUE READING