High-Content Analysis of CRISPR-Cas9 Gene-Edited Human Embryonic Stem Cells

@inproceedings{CarlsonStevermer2016HighContentAO,
  title={High-Content Analysis of CRISPR-Cas9 Gene-Edited Human Embryonic Stem Cells},
  author={Jared Carlson-Stevermer and Madelyn Goedland and Benjamin G. Steyer and Arezoo Movaghar and Meng Lou and Lucille K Kohlenberg and Ryan Prestil and Krishanu Saha},
  booktitle={Stem cell reports},
  year={2016}
}
CRISPR-Cas9 gene editing of human cells and tissues holds much promise to advance medicine and biology, but standard editing methods require weeks to months of reagent preparation and selection where much or all of the initial edited samples are destroyed during analysis. ArrayEdit, a simple approach utilizing surface-modified multiwell plates containing one-pot transcribed single-guide RNAs, separates thousands of edited cell populations for automated, live, high-content imaging and analysis… CONTINUE READING
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