High Ca2+-phosphate transfection efficiency in low-density neuronal cultures

Abstract

This protocol describes a high-efficiency Ca2+-phosphate transfection method with low cell toxicity. The Ca2+-phosphate transfection method is widely used in transfecting neurons because of its low cell toxicity and simplicity in use, but the efficiency is typically low (∼1–5%). To solve this problem we have developed a new Ca2+-phosphate transfection… (More)
DOI: 10.1038/nprot.2006.86

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@article{Jiang2006HighCT, title={High Ca2+-phosphate transfection efficiency in low-density neuronal cultures}, author={Min Jiang and Gong Chen}, journal={Nature Protocols}, year={2006}, volume={1}, pages={695-700} }