• DOI:10.1002/btpr.357
Corpus ID: 24910977

High‐throughput screening techniques for rapid PEG‐based precipitation of IgG4 mAb from clarified cell culture supernatant

@article{Knevelman2010HighthroughputST,
  title={High‐throughput screening techniques for rapid PEG‐based precipitation of IgG4 mAb from clarified cell culture supernatant},
  author={C. Knevelman and J. Davies and L. Allen and Nigel J. Titchener‐Hooker},
  journal={Biotechnology Progress},
  year={2010},
  volume={26}
}

C. Knevelman, J. Davies, +1 author Nigel J. Titchener‐Hooker

Published 2010

Chemistry, Medicine

Biotechnology Progress

Locating optimal protein precipitation conditions for complex biological feed materials is problematic. This article describes the application of a series of high‐throughput platforms for the rapid identification and selection of conditions for the precipitation of an IgG4 monoclonal antibody (mAb) from a complex feedstock using only microliter quantities of material. The approach uses 96‐microwell filter plates combined with high‐throughput analytical methods and a method for well volume… CONTINUE READING

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