High‐throughput screening techniques for rapid PEG‐based precipitation of IgG4 mAb from clarified cell culture supernatant

  title={High‐throughput screening techniques for rapid PEG‐based precipitation of IgG4 mAb from clarified cell culture supernatant},
  author={C. Knevelman and J. Davies and L. Allen and Nigel J. Titchener‐Hooker},
  journal={Biotechnology Progress},
  • C. Knevelman, J. Davies, +1 author Nigel J. Titchener‐Hooker
  • Published 2010
  • Chemistry, Medicine
  • Biotechnology Progress
  • Locating optimal protein precipitation conditions for complex biological feed materials is problematic. This article describes the application of a series of high‐throughput platforms for the rapid identification and selection of conditions for the precipitation of an IgG4 monoclonal antibody (mAb) from a complex feedstock using only microliter quantities of material. The approach uses 96‐microwell filter plates combined with high‐throughput analytical methods and a method for well volume… CONTINUE READING
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