High‐Efficiency RNA Interference in Human Embryonic Stem Cells

@article{Zaehres2005HighEfficiencyRI,
  title={High‐Efficiency RNA Interference in Human Embryonic Stem Cells},
  author={Holm Zaehres and M. William Lensch and Laurence Daheron and Sheila Stewart and Joseph Itskovitz‐Eldor and George Q. Daley},
  journal={STEM CELLS},
  year={2005},
  volume={23}
}
RNA interference methodology suppresses gene expression, thus mimicking loss‐of‐function mutation and enabling in vitro and in vivo gene function analysis. In this study, we used retroviral and lentiviral vectors to deliver small interfering RNAs and report high‐efficiency silencing of a green fluorescent protein (GFP) trans gene and the stem cell–specific transcription factors Oct4/POU5F1 and Nanog in human embryonic stem cells. Gene knockdown of Oct4 and Nanog promotes differentiation… Expand
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