Hhat is a palmitoylacyltransferase with specificity for N-palmitoylation of Sonic Hedgehog.

@article{Buglino2008HhatIA,
  title={Hhat is a palmitoylacyltransferase with specificity for N-palmitoylation of Sonic Hedgehog.},
  author={John A. Buglino and Marilyn D. Resh},
  journal={The Journal of biological chemistry},
  year={2008},
  volume={283 32},
  pages={22076-88}
}
Palmitoylation of Sonic Hedgehog (Shh) is critical for effective long- and short-range signaling. Genetic screens uncovered a potential palmitoylacyltransferase (PAT) for Shh, Hhat, but the molecular mechanism of Shh palmitoylation remains unclear. Here, we have developed and exploited an in vitro Shh palmitoylation assay to purify Hhat to homogeneity. We provide direct biochemical evidence that Hhat is a PAT with specificity for attaching palmitate via amide linkage to the N-terminal cysteine… CONTINUE READING

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