Hexokinase isoenzymes of RIN-m5F insulinoma cells. Expression of glucokinase gene in insulin-producing cells.

@article{Vischer1987HexokinaseIO,
  title={Hexokinase isoenzymes of RIN-m5F insulinoma cells. Expression of glucokinase gene in insulin-producing cells.},
  author={Ulrich M. Vischer and Benigna Blondel and Claes B Wollheim and Wiebke H{\"o}ppner and Hans Joachim Seitz and Patrick B. Iynedjian},
  journal={The Biochemical journal},
  year={1987},
  volume={241 1},
  pages={249-55}
}
We have analysed the pattern of expression of the hexokinase isoenzyme group in RIN-m5F insulinoma cells. Three hexokinase forms were resolved by DEAE-cellulose chromatography. The most abundant isoenzyme co-eluted with hexokinase type II from rat adipose tissue and displayed a Km for glucose of 0.15 mM, similar to the adipose-tissue enzyme. Hexokinase type II was in large part associated with a particulate subcellular fraction in RIN-m5F cells. The two other hexokinases separated by ion… CONTINUE READING