Heterotopic tracheal transplants: techniques and applications.

@article{KleinSzanto1984HeterotopicTT,
  title={Heterotopic tracheal transplants: techniques and applications.},
  author={A. Klein-Szanto and B. C. Pal and Margaret Terzaghi and A. Marchok},
  journal={Environmental Health Perspectives},
  year={1984},
  volume={56},
  pages={75 - 86}
}
Heterotopic tracheal transplants, placed subcutaneously in syngeneic rats have been extensively used in our laboratory. The objective of these experiments was to study the toxic and/or carcinogenic effect of several compounds on the respiratory tract mucosa. This was attained by exposing the transplants to an intraluminal pettet containing the toxicant or carcinogen mixed with an adequate matrix (gelatin, beeswax, stearyl alcohol, silastic, etc.). By varying the concentration of the test… Expand
The use of tracheal implants in toxicology and carcinogenesis research.
TLDR
The advent of molecular pathology techniques such as in situ hybridization will further expand the uses of tracheal implant technology for studies with xenografted human tissues, and should prove useful for the examination of species- and tissue-specific characteristics of growth and differentiation. Expand
Cryopreservation of canine trachea: functional and histological changes.
TLDR
It is concluded that smooth muscle, epithelium with mucus production, and ciliary function were retained after cryopreservation and reimplantation for canine tracheal viability and histological changes were suggestive of early cartilage ischemia. Expand
Pathological Changes Induced by Formaldehyde in Open-Ended Rat Tracheal Implants Preexposed to Benzo(a)pyrene
TLDR
These effects were seen in the tissues as well as in the exfoliated cells, which attest to the latter as an efficient, non-destructive, method for determining the responses of the tracheas to exposure to toxic and carcinogenic agents. Expand
Histogenesis and morphogenesis of epidermoid metaplasia in hamster tracheal organ explant culture
TLDR
The formation of epidermoid metaplasia was studied in hamster tracheal epithelium in long-term serum-free organ expiant culture and similarities and differences between the morphogenesis and histogenesis in vivo and in vitro were emphasized. Expand
A tobacco-specific N-nitrosamine or cigarette smoke condensate causes neoplastic transformation of xenotransplanted human bronchial epithelial cells.
TLDR
It is indicated that cigarette smoke condensate and N-nitrosamine 4-(methylnitroamine)-1-(3-pyridyl)-1-butanone induce in vivo phenotypic changes in BEAS-2B cells similar to the progressive changes that occur during human lung carcinogenesis. Expand
Sequential appearance of anchorage independence, uncontrolled nuclear division and tumorigenicity in 7,12-dimethylbenz(a)anthracene-exposed rat tracheal epithelial cells.
TLDR
Results indicate that anchorageindependence precedes the two other markers of growth autonomy; uncontrolled nuclear division appears as a separate property after anchorage independence and before tumorigenicity; tumorigicity appears preferably in the cell populations that exhibit anchorage independent and uncontrolled nucleardivision. Expand
Oncogene Expression in Cell Lines Derived From Rat Tracheal Implants Exposed In Vivo to 7,12‐Dimethylbenz[a]anthracene
TLDR
The stage‐specific expression of the c‐myc oncogene in these tracheal cell lines suggests a correlation between the regulation of certain oncogenes and neoplastic progression in this model of respiratory carcinogenesis. Expand
[3H]benzo[a]pyrene utilization in rats following tracheal implant exposure.
Open-ended rat tracheal implants (OETI) were exposed to 40 micrograms [3H]benzo[a]pyrene (B[a]P)-gelatin pellets and the 3H activity in the OETI, the host's tissues and excretia was determined 3-96 hExpand
Ha‐ras oncogene mutations in cell lines derived from rat tracheal implants exposed in vivo to 7,12‐dimethylbenz[a]anthracene
TLDR
Restriction fragment‐length polymorphism (RFLP) analysis revealed an A → T transversion in the second base of codon 61 in 2 of 11 cell lines, indicating a lack of correlation between neoplastic stage and ras mutation. Expand
Effects of mutationally activated Ha‐ras on c‐fos expression kinetics in rat tracheal epithelial cells
TLDR
The results suggest that mutations in ras can lead to alterations in nuclear components of the ras signaling pathway at the level of gene transcription. Expand
...
1
2
...

References

SHOWING 1-10 OF 69 REFERENCES
Toxic and tumorigenic effects of asbestos on tracheal mucosa.
TLDR
The studies show that chrysotile causes chronic irritation of the tracheal mucosa, augments sarcoma development, and is a weakly but definitely carcinogenic agent for the respiratory tract mucosa. Expand
Carcinogenicity and cocarcinogenicity of chromium carbonyl in heterotopic tracheal grafts.
TLDR
The data indicate that this metal carbonyl is a carcinogen that can act synergistically with BP and demonstrate the utility of the technique as an efficient tissue-specific bioassay. Expand
Repopulation of denuded tracheal grafts with normal, preneoplastic, and neoplastic epithelial cell populations.
TLDR
The experiments show that this “in vivo culture system” is well suited to the study of growth and differentiation characteristics of carcinogen-altered or preneoplastic epithelial cell populations, and believes that this new in vivo culture approach will effectively complement the existing in vitro culture systems used in studying the process of epithelial carcinogenesis. Expand
Induction of neoplasms in hamster tracheal grafts with 3-methylcholanthrene-coated Lycra fibers.
TLDR
The dosage-dependent sensitivity of the hamster tracheal epithelium to relatively small quantities of 3MC is demonstrated and the direct application of carcinogens coated on synthetic fibers provides a useful tool for assaying water-insoluble carcinogens. Expand
Differentiation of normal and cultured preneoplastic tracheal epithelial cells in rats: importance of epithelial mesenchymal interactions.
TLDR
Observations were consistent with those of other investigators in that normal cell survival and differentiation depend to some extent on interaction with extracellular material(s) present in various organs. Expand
A method for experimental induction of bronchogenic carcinoma in subcutaneously implanted bronchial autograft in dogs.
TLDR
A method for induction of carcinoma in subcutaneously implanted bronchial autografts in dogs revealed atypical metaplastic and proliferative changes further advanced than those seen in the initial biopsy specimens. Expand
Dynamics of neoplastic development in carcinogen-exposed tracheal mucosa.
TLDR
The qualitative and quantitative cellular changes occurring in tracheal epithelium in vivo during the process of carcinogenesis were investigated by use of an in vitro assay which measures the formation of epithelial foci (EF); this method is called EF assay, suggesting the existence of EFFU's endowed with different in vitro growth capacities. Expand
Quantitative exposure of grafted rat tracheas to 7, 12-dimethylbenz(a)anthracene.
TLDR
The lowest DMBA dose presently known to diduce carcinomas in this experimental model is 40 microng, which is in the dose range used for tumor initiation in skin carcinogenesis studies in mice. Expand
Induction of preneoplastic and neoplastic lesions in grafted rat tracheas continuously exposed to benzo(a)pyrene.
TLDR
This experimental model appears to be well suited for the study of the sequential epithelial changes that lead to respiratory tract neoplasia, since a variety of metaplastic and dyplastic lesions can be induced by carcinogenic polycyclic hydrocarbons in the transplanted rat tracheas. Expand
Reestablishment of a mucociliary epithelium in tracheal organ cultures exposed to retinyl acetate: a biochemical and morphometric study.
TLDR
Tracheal explants derived from vitamin A-deficient rats underwent keratinizine squamous metaplasia in organ culture when grown in serum-free medium and found that vitamin A had a greater effect on the incorporation of [3H]glucosamine than on [14C]serine into the secreted mucins, particularly at the higher retinyl acetate concentrations. Expand
...
1
2
3
4
5
...