Heterotetramers formed by an S-layer-streptavidin fusion protein and core-streptavidin as a nanoarrayed template for biochip development.

@article{Huber2006HeterotetramersFB,
  title={Heterotetramers formed by an S-layer-streptavidin fusion protein and core-streptavidin as a nanoarrayed template for biochip development.},
  author={Carina Huber and Jing Liu and Eva Maria Egelseer and Dieter Moll and Wolfgang Knoll and Uwe Sleytr and Margit S{\'a}ra},
  journal={Small},
  year={2006},
  volume={2 1},
  pages={142-50}
}
Based on the S-layer protein SbpA of Bacillus sphaericus CCM 2177, an S-layer-streptavidin fusion protein was constructed. After heterologous expression, isolation of the fusion protein, and refolding, functional heterotetramers were obtained that had retained the ability to recrystallize into the square-lattice structure on plain gold chips and on gold chips precoated with secondary cell wall polymer (SCWP), which is the natural anchoring molecule for the S-layer protein in the bacterial cell… CONTINUE READING
Highly Cited
This paper has 25 citations. REVIEW CITATIONS

Similar Papers

Loading similar papers…