Heteronuclear transfers from labile protons in biomolecular NMR: Cross polarization, revisited.

@article{Novakovic2021HeteronuclearTF,
  title={Heteronuclear transfers from labile protons in biomolecular NMR: Cross polarization, revisited.},
  author={Mihajlo Novakovic and Sundaresan Jayanthi and Adonis Lupulescu and Maria Grazia Concilio and Jihyun Kim and Dave Columbus and Ilya Kuprov and Lucio Frydman},
  journal={Journal of magnetic resonance},
  year={2021},
  volume={333},
  pages={
          107083
        }
}

References

SHOWING 1-10 OF 55 REFERENCES

Hadamard magnetization transfers achieve dramatic sensitivity enhancements in homonuclear multidimensional NMR correlations of labile sites in proteins, polysaccharides and nucleic acids

This study demonstrates that even larger sensitivity gains per unit time, equivalent to reductions by several hundred-folds in the duration of experiments, can be achieved by looping inversion or using saturation procedures.

Solution-State NMR Experiments Based on Heteronuclear Cross-Polarization

Heteronuclear coherence transfer in liquid-state NMR applications has been traditionally performed using pulse-interrupted delay schemes such as INEPT-type pulse trains. So far, the alternative use

Confronting the Invisible: Assignment of Protein 1HN Chemical Shifts in Cases of Extreme Broadening.

Applications to the CAPRIN1 protein in both dilute- and phase-separated states highlight the utility of the method, establishing that accurate 1HN chemical shifts can be obtained even in cases where solvent hydrogen exchange rates are on the order of 1500 s-1.

Influence of Chemical Exchange among Homonuclear Spins in Heteronuclear Coherence-Transfer Experiments in Liquids

Abstract An analysis is presented of the effects of chemical exchange on the coherence-transfer efficiencies of two different types of solution-state NMR methodologies, represented by the

The Incorporation of Labile Protons into Multidimensional NMR Analyses: Glycan Structures Revisited

This study shows that even larger enhancements can be obtained for rapidly exchanging OHs in saccharides, leading to the retrieval of previously undetectable 2D TOCSY/NOESY cross-peaks with nonlabile protons.

Magnetization Transfer to Enhance NOE Cross‐Peaks among Labile Protons: Applications to Imino–Imino Sequential Walks in SARS‐CoV‐2‐Derived RNAs

This study introduces an alternative that also enhances NOEs between such labile sites, based on encoding a priori selected peaks by selective saturations, and the resulting selective magnetization transfer (SMT) experiment proves particularly useful for enhancing the imino–imino cross‐peaks in RNAs.

A complete set of novel 2D correlation NMR experiments based on heteronuclear J-cross polarization

The combination of the echo/anti-echo approach, heteronuclear gradient echoes and the preservation of equivalent pathways (PEP) methodology affords a general approach to obtain sensitivity-enhanced pure-absorption 2D spectra that can be used as interesting alternatives to conventional pulse-interrupted free-precession INEPT-based pulse schemes, such as HSQC-type and TROSY-type experiments.
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